Venturing into the realm of urban legend, the 4:44 ritual involves manipulating radio waves near the mystical Akeyuki Sacred Tree at the precise time of 4:44 to access a different dimension. When Asuka Tsuchimiya and her companions—Nana Nanase, Mia Silverstone, Yuu Tounaka, and Chloe Morisu—embark on this experiment through the Crystal Radio Research Club, they are astounded by its success. Transported to a parallel world, referred to as a fragment, they encounter a strangely familiar figure—Asuka's counterpart named Seriouska, distinguished by her serious demeanor and combat prowess. Seriouska discloses a looming threat known as the Twilight, which threatens to eradicate all potential across parallel realms. To thwart the malevolent Twilight King orchestrating this chaos, Seriouska enlists the group's help. As the narrative unfolds in Akanesasu Shoujo, the girls grapple with embracing their authentic selves while pursuing the enigmatic Twilight King. Little do they realize, the key to vanquishing the encroaching Twilight may be within their reach.