When Subaru's basketball dreams hit a roadblock, he abandons the sport until his aunt enlists him to coach the Keishin Academy girls' basketball team. Reluctantly taking on the task, Subaru guides Tomoka, Maho, Airi, Hinata, and Saki to hone their skills and take on formidable rivals. Ro-Kyu-Bu! charts the journey of these young girls and their coach as they refine their unique and adorable basketball prowess.