Chizuru Yukimura, on a quest to find her missing father in Kyoto, joins forces with the Shinsengumi after a harrowing encounter with a bloodthirsty creature. As the group relocates to Nishi Honganji Temple for their city patrols, rumors surface about impostor bandits wreaking havoc in their name. With the imminent arrival of the Shogun Iemochi in Kyoto, the Shinsengumi is tasked with his protection, prompting urgent investigations led by Hijikata. Amidst the chaos, a mysterious figure resembling Chizuru aids in the escape of dangerous rasetsu, leading to her disappearance alongside Sakai, the treasurer. As the Satsuma domain alerts of an assassination plot by Tosa domain ronin, Kazama and his cohorts journey to Kyoto. With tensions escalating and time running short, the Shinsengumi grapples with a series of interconnected incidents, forcing Hijikata and his comrades to make pivotal decisions before the Shogun's arrival.