Upon transferring to an all-girls school in search of love, Kanako Miyamae encounters the captivating Mariya Shidou. However, her romantic hopes shatter when she discovers that Mariya is actually a boy who cross-dresses. Forced to live with Mariya as her roommate, Kanako's days are filled with unfortunate events. Despite longing for her ideal girl, Kanako must navigate the devious tricks of the sadistic Mariya and his sharp-tongued maid, Matsurika Shinouji. In Maria†Holic Alive, Kanako continues her quest for love while grappling with the challenges of high school life and her peculiar living situation with Mariya.